The intracellular uptake of 6-carboxyfluorescein (6-FAM)-labelled noncoding siRNA (FAM-siRNA), ... Fura-2 is a larger fluorophore with a much greater extinction coefficient and a higher quantum efficiency translating to 30-fold higher brightness per molecule (Cobbold & Rink, 1987). Add to cart. In addition to its relatively high absorptivity, excelle Das Fab-Fragment, auch Fab-Antikörper (von engl.Fragment antigen binding), ist das Antigen-bindende Fragment eines Antikörpers, das durch Spaltung mit dem Enzym Papain gewonnen werden kann. For accurate quantification, it is important to determine the hypochromicity of the oligonucleo-tide or complex nucleic acid structure. … Build My Oligo. Fluorescein as a 5' modification; MW: 538 g/mol: Excitation maximum: 495 nm: Emission maximum: 520 nm: Extinction coefficient (260nm) 13.7 mM-1 cm-1: Fluorescein as a 3' modification; … Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Extinction coefficient (M –1 cm –1) QY †† Cyanine5 dye [Cy5] 646: 662: 250,000: 0.3: 6-FAM [FAM] 495: 521: 75,000: 0.9: HEX [HEX] 537: 556: 96,000: 0.7: TET [TET] 519: 539: 86,000: 0.9 †† QY (quantum yield) is a calculated ratio between the number of photons emitted and the number of photons absorbed. Fluorescence Maximum: 520. Categories: < 650 nm emission fluorescent probes, RIS & its analogue probes SKU: BV110101. Photostable dyes suitable for microscopy Perfect match for the CFP filter set Highly water soluble and pH-insensitive Suitable for flow cytometry in the AmCyan channel. Please use OLIGObuilder™ to generate pricing and order or contact us to speak with a customer service representative. CAS No. Emission (nm): 492 / 519 nm. If no extinction coefficient information exists for a protein or protein mixture of interest, and a rough estimate of protein concentration is required for a solution that has no other interfering substances, assume εpercent = 10. : 1093621-79-4. The extinction coefficient of a protein therapeutic is usually determined experimentally during early product development and has some inherent method variability. Fluorescein is a fluorescent dye consisting of two isomers, one of them being 6-Fam (6-carboxyfluorescein). Product usage: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 816.7. Ready to order your oligonucleotide? Carboxyfluorescein (FAM) is another commonly used fluorescein derivative and its advantages include convenient synthesis, stability in water and so on. Extinction coefficient: 40,000 Molecular weight: ~716 Excitation laser line: 405 nm Replaces: Alexa Fluor® 430. An algorithm for this calculation can be found on the web. FAQs . Click here. Key to the Beer-Lambert law is the extinction coefficient, which measures how strongly the absorbing bases reduce the intensity of the UV light from incidence to transmission. Extinction Coefficient at Lambda max: 54700. The succinimidyl ester of 5-FAM ... Rhodamine Green dye offers a combination of desirable properties, including good photostability, a high extinction coefficient (>75,000 cm-1 M-1) and a high fluorescence quantum yield, particularly in its nucleotide and nucleic acid conjugates. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. As shown in Fig. Scale of light extinction coefficient for fertilizer treatment control, 50, 100, 150 kg ha-1, was 0.4675, 0.4794, 0.4858 and 0.495, respectively and for Pishtaz, Sepahan and SW-486 cultivars 0.488, -0.4618 and -0.4504, respectively. The salt concentration was gradually increased to 100 mM as described above. extinction coefficients weighted by the number of times each base appears in the sequence.6 Both methods do not account for potential hypochromicity in the oligonucleotide or complex nucleic acid structures. Molecular Weight: 831.71. A spectrally unique, 405 nm-excitable green dye. Features. FAM Amine is an unreactive functional group that induces amide bond with carboxyl group in substrate of labeling target through appropriate catalytic reaction. Den alternativen Namen Resorcinphthalein verdankt der Stoff den Reaktanten, aus denen ihn Adolf von Baeyer synthetisierte.Er mischte Phthalsäureanhydrid (auch Phthalsäure ist verwendbar) mit Resorcin und fügte konzentrierte Schwefelsäure als wasserabspaltendes Mittel hinzu. So we ignored the Cystine effect, since Cysteine is also a rare amino acid (~2% in the average protein). 6-FAM Amidite - (O-0331) --> Skip to the beginning of the images gallery. Description Description. EXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of occurrences. Or use a formula that takes into account nearest neighbor effects. Most protein extinction coefficients (ε percent) range from 4.0 to 24.0. 260 = extinction coefficient of the oligonucleotide at 260 nm in L ⋅ mol-1 ⋅ cm-1. For more than 30 years, we have provided leading labs, universities and medical organizations with the finest scientific instruments for research and clinical applications. Product Pricing Order; 100 nmole DNA Oligo: $60.00 USD: Add To Order: 250 nmole DNA Oligo: $75.00 USD: Add To Order: 1 umole DNA Oligo: $155.00 USD: Add To Order : 2 umole DNA Oligo: $201.50 USD: Add To Order: 5 umole DNA Oligo: $375.00 USD: … CF280: 0.2. Extinction Coefficients Dye IgG VivoTag-S 680 Epsilon 220,000 A/M 210,000 A/M Absorbance 670 (A670) Crosstalk to A280 16% VivoTag-S 750 Epsilon 240,000 A/M Absorbance 280 is 6% of absorbance 750. ISS is a leading manufacturer of fluorescence and biomedical instrumentation. c = concentration in mol L-1. : Maximum absorption wavelength (λmax (abs)): 493 nm . the ‘fluorophore’ and the ‘quencher’. The NHS ester … ℓ = pathlength in cm. Product Details . Radiation interception, extinction coefficient and use efficiency of wheat crop at various irrigation and nitrogen levels in a semi-arid location S. Pradhan1,2 • V. K. Sehgal2 • K. K. Bandyopadhyay2 • P. Panigrahi1 • C. M. Parihar2 • S. L. Jat3 Received: 23 October 2016/Accepted: 17 September 2018/Published online: 29 September 2018 The Author(s) 2018 Abstract Field experiments wer Extinction coefficient (M-1 cm-1): 73,000 (0.1 M phosphate buffer, pH 7.2) size: Clear: 5-FAM-RIS quantity. Extinction coefficient Filter Accell Green 6-FAM (494 nm /520 nm) 75,000 M–1 cm–1 FITC Accell Red DY-547 (557 nm /570 nm) 150,000 M–1 cm–1 Cy 3, Rhodamine and PE Table 3.